Inoculation, fermentation, extraction (MeOH & CH2Cl2), Kupchan, semi-preparative HPLC
Inoculation, fermentation, extraction(MeOH), fractionation (SPE column), semi-preparative HPLC.
Inoculation, Fermentation, Solid Phase Extraction, Molecular Network analysis of fractions, Disc diffusion assay, and HPLC.
Inoculation, fermentation, extraction (MeOH), fractionation using silica gel 60, and HPLC-UV Analysis.
Column chromatography and purified using Sephadex LH-20 column.
The ethanol extract was partitioned, and the ethyl acetate fraction was subjected to silica gel column and further purified using Sephadex LH-20 column
Extraction by percolation using acetone, column chromatography and preparative HPLC.
The compound was isolated from the petroleum ether fraction of the roots of Piper guineenses
Cold maceration, column chromatography and semi-preparative HPLC.